Table 3

Primers

PrimerNameSequence
P1Sso1354 with native promoter SphI ForwardTAGATAGTGCATGCGAATTATCCAGTCCTCTATTTCTCTTACG
P2Sso1354 C-terminal 6Ă—His tag SphI ReverseATCTAGCTGCATGCTCAGTGGTGGTGGTGGTGGTGGAGGAGAGTTTCAGAAAAGTTGGA
P3Sso1303 Sso1388 ForwardTTGGCTTAAAGTATAGCCTTTA
P4Sso1303 Sso1388 ReverseCTGCTCTGCTTTGAAGATTCT
P5Sso3052 SacI ForwardCATCAGAGCTCCAAACAACGCTCCTTTAGGG
P6Sso3052 BamHI ReverseATCCAGGATCCTTATTCTGGTGCGTAGACGTTAGG
P7Sso3060 PstI ForwardCATCACTGCAGCTATCTATAATCTAGGATAACCCTTATAAC
P8Sso 3060 SphI ReverseATCCAGCATGCTATCCCATTTAGGTGATGTTGTTAG
P9Sso2833 SacI ForwardCATCAGAGCTCAGCACAAGGGTTTTCCCTTT
P10Sso2833 BamHI ReverseATCCAGGATCCTCATACCTTAAAGTTTAATATCGTCCA
P11Sso2850 PstI ForwardCATCACTGCAGTTAGCAATTTAGATGCCAGGATG
P12Sso2850 SphI ReverseATCCAGCATGCAGAATGTATCGGATGATCAGAATACG
P13Sso3053 internal ForwardAACAATACGCTAATTTAACGAATCC
P14Sso3053 internal ReverseATTCCTTTAGCTAGAAGGCCATATG
P15Sso3053 SphI ForwardAGTCAGCATGCCCATAAATATAAAAGTTTCGCCATTT
P16Sso3053 SphI ReverseGTACAGCATGCTTATCTTCTTCTTACCACTACATAAGCTACT
P17Sso1354 internal ForwardATCATTTACTTGCATCAACA
P18Sso1354 internal ReverseTTAGAGGAGAGTTTCAGAAAAGTTGG
P19Sso2847 internal ForwardTACCCAGCAAAAGGATGACC
P20Sso2847 internal ReverseCCTCCGTTGCCTCAATACAT