Table 3

Changes in expression levels of TCA cycle genes during nitrogen depletion in cultures in minimal medium with fructosea

GeneLog2 expression level
Fructose growthPHB production
Citrate synthase11.89.5
Aconitase10.88.7
Isocitrate dehydrogenase10.49.5
α-Ketoglutarate dehydrogenase
    odhA11.511.0
    odhB10.710.3
    odhL11.99.8
Succinyl-CoA synthetase
    sucC12.28.2
    sucD11.96.6
Succinate dehydrogenase
    sdhA12.110.0
    sdhB12.610.4
    sdhC13.111.0
    sdhD13.812.0
Fumarase11.28.8
Malate dehydrogenase11.28.3
  • a All P values were <0.01, except for odhB (P value of 0.04).