Table 1

Strains and plasmids used in this study

Strain or plasmidGenotype or characteristicReference
Strains
    R. eutropha
        H16Wild type; Genr68
        Re2411H16 Δ(H16_A1337) (ΔrelA)This study
        Re2424H16 Δ(H16_A2373) (ΔrpoS)This study
        Re2061H16 ΔphaCABThis study
    E. coli S17-1Strain for conjugative transfer of plasmids into R. eutropha54
Plasmids
    pJV7phaC deletion plasmid; confers kanamycin resistance12
    pBBR1MCS-2Broad-host-range cloning vector; confers kanamycin resistance27
    pDS1spoT2 deletion plasmid; confers kanamycin resistanceThis study
    pCB300rpoS deletion plasmid; confers kanamycin resistanceThis study
    pCJB-CABphaCAB deletion plasmid; confers kanamycin resistanceThis study
    pASCB1pBBR1MCS-2 with R. eutropha rpoS gene inserted into the multiple-cloning siteThis study