Table 1

Strains and plasmids used in this work

Strain or plasmidDescriptionSource or reference
Strains
    B. thuringiensis subsp. kurstaki
        ATCC 33679HD-1 biopesticide strainATCCa
        T1B1ATCC 33679 ΔpHD1-XO1; barcoded at target 1 with common tag and specific tag 1This work
        T1B2ATCC 33679 ΔpHD1-XO1; barcoded at target 1 with common tag and specific tag 2This work
        ForayCommercial HD-1 biopesticide product dispersed in Fairfax County, VA45
    E. coli
        SM10E. coli donor strain24
        SCS110pSS4333 donor strain24
Plasmids
    pRP1028Allelic exchange vector, turbo-rfp, Spcr24
    pSS4332I-SceI expression vector, gfp, Kanr24
    pT1B1pRP1028 containing target 1 with common tag and specific tag 1DNA2.0
    pT1B2pRP1028 containing target 1 with common tag and specific tag 2DNA2.0
  • a ATCC, American Type Culture Collection.