Table 5

Specificity of real-time PCR assays for B. thuringiensis subsp. kurstaki strains

Strain or material testedSpecificitya
Common tagSpecific tag 1Specific tag 2
B. thuringiensis subsp. kurstaki0/40/40/4
B. thuringiensis subsp. kurstaki T1B14/4 (22.0)4/4 (25.2)0/4
B. thuringiensis subsp. kurstaki T1B24/4 (27.2)0/44/4 (22.0)
Bacillus anthracis VNR-Δ10/40/40/4
B. anthracis Ames0/40/40/4
B. anthracis NNR-Δ10/40/40/4
B. anthracis ΔSterne0/40/40/4
B. thuringiensis subsp. israelensis ATCC 356460/40/40/4
Bacillus cereus HER14140/40/40/4
B. subtilis ATCC 273700/40/40/4
B. atrophaeus subsp. globigii0/40/40/4
Pseudomonas aeruginosa PAO-10/40/40/4
Streptococcus pyogenes ATCC 123840/40/40/4
Bordetella pertussis ATCC 97970/43/4 (39.0)b0/4
Salmonella enterica serovar Typhimurium ATCC 140280/40/40/4
Escherichia coli ATCC 439850/40/40/4
Human placental DNA0/41/4 (39.8)b0/4
Escherichia coli O157:H70/40/40/4
Francisella tularensis SHU40/40/40/4
Yersinia pestis HARBIN350/40/40/4
  • a Number of samples crossing threshold per number of replicates tested. One nanogram of genomic DNA was tested per replicate. Values in parentheses are average CT values.

  • b Negative dissociation curve data.