Table 2

Primers and probes selected for qPCR quantification and reaction efficiency

SpeciesTarget genePrimers and probes (5′→3′)aqPCR efficiency (%)R2
K. lactislac4Primer 1: GCG CAC GGA TCT GTA ACA ATC101.40.994
Primer 2: GTT TAT CGA CAA GGA CAC GGG
Probe: 5′FAM-CAA ATA AGC ACG ACT TCA TTA CGA CAG ACC ACT T-TAMRA3′
D. hanseniipik1Primer 1: AGA GCG CCG ATA GTA CTT CTA AGG100.40.995
Primer 2: AAG CTT GGA CAC ATG GGA AGA
Probe: 5′FAM-ACT GCG CAA GCT TCA AAA TTC GGC TA-TAMRA3′
G. candidumcglPrimer 1: CGC TTT CAC CAA CTC CAT TGA93.10.995
Primer 2: CTT GTC TGG ATC GAG TCT CCC T
Probe: 5′FAM-CCG AGC TCC GTG ACA TCA TCA CTG A-TAMRA3′
P. camembertitubPrimer 1: CCG TTC TCG TCG ATT TGG A94.30.993
Primer 2: CTT TCG GCA AGC TTT TCC G
Probe: 5′FAM-TGG TAC CAT GGA CGC TGT CCG CT-TAMRA3′
  • a FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.