Table 1

Effect of Triton-X on L. pneumophila cultivability and amoeba lysisa

Time (min)Triton-X concentration (%)% of cells lysedb% of cultivable L. pneumophila cellsc
A. polyphagaN. fowleri
MeanSDMeanSDMeanSD
00.02100.0±0.00
0.01100.0±0.00
40.02100.5±1.56
0.0197.4±3.72
60.0211.6±9.36
0.0190.5±1.13
80.020.35±0.20
0.0192.6±1.87
10No Triton-X0.0±2.200.0±0.63100.0±0.00
0.0291.2±0.93100±0.000.06±0.04
0.010.1±1.364.1±1.6395.4±1.47
  • a Data are from 3 independent experiments. Cells were incubated in either 0.22-μm-filtered drinking water (ftH2O) or Triton-X for 0 to 10 min at room temperature.

  • b Amoebae were counted using a hemacytometer. —, no data.

  • c Numbers of CFU were enumerated as described previously.