Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Microalgae | ||
| Chorella sorokiniana strain RP | Isolated from freshwater ponds in Buenos Aires, Argentina, in 2009 | Laboratory collection |
| Scenedesmus obliquus strain C1S | Isolated from freshwater ponds in Buenos Aires, Argentina, in 2009 | Laboratory collection |
| Pseukirchneriella sp. strain C1D | Isolated from freshwater ponds in Buenos Aires, Argentina, in 2009 | Laboratory collection |
| Azotobacter vinelandii | ||
| DJ | Wild type | D. Dean |
| AV2 | ΔnifA::Sp | This study |
| AV3 | ΔnifL | This study |
| UW211 | nifHp-lacZ | 14 |
| UW218 | ΔnifA::Sp nifHp-lacZ | 14 |
| UW226 | ΔnifL nifHp-lacZ | This study |
| Escherichia coli DH5α | F′ endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK− mK+) λ− | 34 |
| Plasmids | ||
| pUK21 | Cloning vector | 37 |
| pRHB138 | Source of A. vinelandii ΔnifA::Sp allele | 14 |
| pRHB142 | Partial construction of A. vinelandii ΔnifL allele | This study |
| pRHB143 | Source of A. vinelandii ΔnifL allele | This study |