Table 1

Bacterial strains used in this study

StrainaRelevant phenotypebSource or reference
Escherichia coli K-12 DH5αNonpathogenic; Gram negative; Amps17
Escherichia coli K-12 DH5α(pPCR-Script Amp SK+)Contains high-copy-number plasmid with β-lac; Gram negative; AmprThis study
Escherichia coli O157:H7Pathogen; Shiga toxin, STEC; Amps35
Escherichia coli O91:H21Pathogen; Shiga toxin, STEC; Amps22
Vibrio cholerae THR7000Nontoxigenic mutant; Gram negative; Amps21
Pseudomonas aeruginosaMultiple drug resistant; Gram negative; AmprWMUc
Enterobacter aerogenesMultiple drug resistant; Gram negative; AmprWMU
Staphylococcus aureus ST398Methicillin resistant; Gram positive; Ampr39
  • a E. coli serotypes O157:H7 and O91:H21 are pathogenic clinical isolates that produce Shiga toxin and cause the hemolytic-uremic syndrome.

  • b Amps, ampicillin sensitive; Ampr, resistant to ampicillin and β-lactamase positive.

  • c WMU, Western Michigan University Bacterial Stock for Teaching Laboratory.