Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristicsaReference or source
Strains
    Escherichia. coli
        EC100D+Epicentre Technologies
        S17-1λ pir/pMiniHimar RB1pMiniHimar RB1 transposon vector in S17-1λ pir; Kmr2
    Dickeya dadantii
        3937Wild type, Saintpaulia (African violet) isolateN. Hugouvieux-Cotte-Pattat
        WPP92hrpY::Kan; 3937 derivative; Kmr40
        WPP67hrpX:aadA; 3937 derivative; Spr Smr40
        Ech3049slyA::Kan; Himar RB1 transposon mutant; KmrThis work
        Ech4120slyA::Kan; Himar RB1 transposon mutant; KmrThis work
        Ech4380slyA::Kan; Himar RB1 transposon mutant; KmrThis work
        Ech4396slyA::Kan; Himar RB1 transposon mutant; KmrThis work
        Ech163slyA::Kan; deletion mutant; 3937 derivative; KmrThis work
        Ech164expI::Kan; deletion mutant; 3937 derivative; KmrThis work
        Ech165expR::Kan; deletion mutant; 3937 derivative; KmrThis work
        Ech166Chromosomal insertion of lacY-slyA-cm-prt in Ech163; Kmr CmrThis work
        Ech188hrpS::Kan; deletion mutant; 3937 derivative; KmrThis work
Plasmids
    pPROBE-ATPromoter-GFP reporter plasmid; Apr21
    pKD4Template plasmid carrying Kan cassette; Kmr8
    pGEM-T easyCloning vector; lacZ; AprPromega Corp.
    pPhrpShrpS promoter region cloned in pPROBE-AT; Apr34
    pPhrpLhrpL promoter region cloned in pPROBE-AT; Apr34
    pPhrpAhrpA promoter region cloned in pPROBE-AT; AprThis work
    pPhrpNhrpN promoter region cloned in pPROBE-AT; AprThis work
    pPslyAslyA promoter region cloned in pPROBE-AT; AprThis work
    pPhrpXhrpX promoter region cloned in pPROBE-AT; AprThis work
    pPhrpYhrpY promoter region cloned in pPROBE-AT; AprThis work
    pML123RSF1010-derived expression and lac-fusion broad-host-range vector, Gmr16
    pWM91Suicide vector; oriR6K mobRP4 lacZa (of pBluescript II) sacB; Sucr Apr20
    pWM91slyA::Kan3.7-kb fragment containing slyA::Kan in pWM91This work
pWM91expI::Kan3.0-kb fragment containing expI::Kan in pWM91This work
    pWM91expR::Kan3.2-kb fragment containing expR::Kan in pWM91This work
    pMLslyA1.5-kb slyA with native promoter region cloned in pML123; GmrThis work
    pMLhrpL1.6-kb hrpL with native promoter region cloned in pML123; GmrThis work
    pTCLS-Cm6.4-kb lacY-cm-prt region cloned in pGEM-T Easy15
    pTCLSslyA-Cm1.5-kb slyA with its promoter region cloned in pTCLS-Cm; Apr CmrThis work
  • a Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Gmr, gentamicin resistance; Spr, streptomycin resistance.