Table 3

Promoter activities of hrpX and hrpY in D. dadantii 3937 and Ech163 and of slyA in 3937, WPP67, and WPP92

Gene promoteraMean fluorescence intensityb
6 h12 h24 h
3937(pPslyA)119.5 ± 7.3131.7 ± 3.3128.1 ± 2.7
WPP67(pPslyA)119.1 ± 2.4133.7 ± 0.8140.7 ± 0.8
WPP92(pPslyA)127.5 ± 5.4128.5 ± 0.5134.3 ± 5.3
3937(pPROBE-AT)2.6 ± 0.52.1 ± 0.13.4 ± 0.6
3937(pPhrpX)10.8 ± 0.514.3 ± 0.814.8 ± 0.7
Ech163(pPhrpX)11.0 ± 0.212.0 ± 0.413.5 ± 0.9
3937(pPhrpY)25.4 ± 1.035.2 ± 1.134.3 ± 0.9
Ech163(pPhrpY)24.8 ± 0.629.5 ± 0.828.6 ± 0.6
3937(pPROBE-AT)2.3 ± 0.12.6 ± 0.34.4 ± 0.6
  • a WPP67 and WPP92 are hrpX and hrpY mutants of D. dadantii 3937, respectively. Ech163 is the slyA mutant of D. dadantii 3937.

  • b Similar results have been observed in two individual experiments with three biological replicates, and the results of one experiment have been shown here. No significant difference in slyA promoter activity was observed among the wild-type strain (3937), WPP67, WPP92, and Ech163 (P > 0.05, Student's t test).