Table 3

Luciferase expression in response to oxidative stress and cell envelope-active antimicrobial agents

Stimulus or stressor (concn)Luciferase activity ratio (RLU)a
Hydrogen peroxide (0.4 mM)2.09 ± 0.33d
Methyl viologen (10 mM)2.07 ± 0.55c
SDS (8 μg/ml)2.51 ± 0.50d
Chlorhexidine (1.5 μg/ml)3.03 ± 0.42d
Vancomycin (0.75 μg/ml)1.41 ± 0.03b
Bacitracin (20 μg/ml)2.24 ± 0.13b
Nisin (10 μg/ml)2.25 ± 0.47c
d-Cycloserine (20 μg/ml)1.62 ± 0.31c
Penicillin G (0.04 μg/ml)1.66 ± 0.35b
  • a Data are the ratios (average ± standard deviation) of the luciferase activity (in RLU) at the conditions specified to that of controls that received an equal amount of solvent instead of the stressor indicated.

  • b Difference between the groups at the significance level of P < 0.05.

  • c Difference between the groups at the significance level of P < 0.01.

  • d Difference between the groups at the significance level of P < 0.001.