Table 4

Selected genes identified by DNA microarray analysis

Unique IDaDescription/putative functionbArray ratiocqPCR ratiocP value
SMU.246Glycosyltransferase N-acetylglucosaminyltransferase, RgpG−2.6−4.05.9E-3
SMU.549Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase, MurG−2.7−2.03.6E-3
SMU.599d-Alanine-d-alanine ligase, DdlA−1.8−3.27.1E-3
SMU.1677UDP-N-acetylmuramoylananine-d-glutamate-2,6-diaminopimelate ligase, UDP-MurNac-tripeptide synthetase, MurE−13.1−2.73.6E-3
SMU.1689d-Alanyl carrier protein, DltC3.4ND6.9E-3
SMU.1691d-Alanine-d-alanyl carrier protein ligase, DltA3.4ND3.7E-5
SMU.1838Preprotein translocase subunit SecA−1.9−2.06.6E-3
SMU.1948Preprotein translocase subunit SecE−2.8ND2.1E-3
SMU.2006Preprotein translocase SecY protein3.91.91.6E-5
  • a ID, identification.

  • b Descriptions and putative functions of the identified genes are based upon the published S. mutans database.

  • c Levels of expression in the BrpA-deficient mutant relative to those of the wild type, as shown by DNA microarray analysis (array ratio) and real-time PCR (quantitative PCR [qPCR]), with negative numbers indicating downregulation. ND, not done.