Table 1

Primers used in this study

Target gene and primer directionPrimer sequence (5′→3′)Amplicon length (bp)Annealing temp (°C)
foxA
    ForwardGGT TCC TTG AGC GTA TTG ATG1,09458
    ReverseGGT CAT CGG TTT CAG CAG TTT
ail
    ForwardTAA TGT GTA CGC TGC GAG35157
    ReverseGAC GTC TTA CTT GCA CTG
ystA
    ForwardATC GAC ACC AAT AAC CGC TGA G7961
    ReverseCCA ATC ACT ACT GA CTT CGG CT
ystB
    ForwardGTA CAT TAG GCC AAG AGA CG14661
    ReverseGCA ACA TAC CTC ACA ACA CC
yadA
    ForwardCTT CAG ATA CTG GTG TCG CTG T84960
    ReverseATG CCT GAC TAG AGC GAT ATC C759a
virF
    ForwardGGC AGA ACA GCA GTC AGA CAT A56163
    ReverseGGT GAG CAT AGA GAA TAC GTC G
  • a Amplicon length in pathogenic Y. enterocolitica serotype O:8.