Table 1

List of samples from which amoebae and/or mycobacteria were isolateda

Sample locationSample typebAmoeba(e)Mycobacterium species
Patient room 1, shower drainBAcanthamoeba sp. genotype T4
Patient room 1, sink drainBH. vermiformisM. chelonae
Patient room 2, showerhead swabBH. vermiformisM. gordonae
Patient room 2, sink drainBH. vermiformis and Acanthamoeba sp. genotype T4cM. chelonae
Patient room 2, shower drainBH. vermiformis and Acanthamoeba sp. genotype T4M. peregrinum
Patient room 3, sink drainBM. peregrinum
Patient room 3, sink waterWH. vermiformisM. gordonae
Patient room 3, shower drainB2 different Acanthamoeba species, genotypes T3 and T4Zielh +
Patient room 4, sink faucet headBM. gordonae
Patient room 4, sink drainBH. vermiformis and Flamella sp.Zielh +
Patient room 4, sink waterWM. gordonae
Patient room 4, showerhead swabBH. vermiformis
Patient room 4, shower drainBAcanthamoeba sp. genotype T3Zielh +
Patient unit, water fountain faucet headBAcanthamoeba sp. genotype T4M. gordonae
Patient unit, kitchen ice/water machine drainBDistantly related to Allovahlkampfia sp.M. peregrinum
Hospital therapy pool tile floorBM. gordonae
Pool filter 1 (end of pool)BM. mucogenicum
Pool water dispenserBAcanthamoeba sp. genotype T4
Research building, water fountain dispenserBM. peregrinum and M. gordonae
Enzymatic cleaner containerBM. gordonae
Diagnostic center sink, running waterWM. avium and M. gordonae
  • a We mention “Ziehl +” only when acid-fast bacteria were observed after amoebal coculture but could not be recovered on 7H10 plates.

  • b B, biofilm; W, water.

  • c This isolate was successfully grown in PYG broth without bacteria and is termed “Acanthamoeba H16” in the text.