Gene | Primer | Sequence (5′–3′) | Cycling conditions | Amplicon size (bp) | Reference |
---|---|---|---|---|---|
wzx | wzxO26-F | CAGAATGGTTATGCTACTGT | 30 cycles of 20 s at 95ï¿½C, 40 s at 60 to 54ï¿½C, and 30 s at 72ï¿½C | 423 | 13 |
wzxO26-R | CTTACATTTGTTTTCGGCATC | ||||
WzxO103-F | TTGGAGCGTTAACTGGACCT | 30 cycles of 20 s at 95ï¿½C, 40 s at 57ï¿½C, and 30 s at 72ï¿½C | 321 | 13 | |
wzxO103-R | GCTCCCGAGCACGTATAAG | ||||
wzxO111-F | TAGAGAAATTATCAAGTTAGTTCC | 30 cycles of 20 s at 95ï¿½C, 40 s at 62ï¿½C, and 30 s at 72ï¿½C | 406 | 13 | |
wzxO111-R | ATAGTTATGAACATCTTGTTTAGC | ||||
wzxO145-F | CCATCAACAGATTTAGGAGTG | 30 cycles of 20 s at 95ï¿½C, 40 s at 59ï¿½C, and 30 s at 72ï¿½C | 609 | 13 | |
wzxO145-R | TTTCTACCGCGAATCTATC | ||||
wzxO157-F | CGGACATAAATGTGATATGG | 30 cycles of 20 s at 95ï¿½C, 40 s at 60ï¿½C, and 30 s at 72ï¿½C | 259 | 13 | |
wzxO157-R | TTGCCTATGTACAGCTAATCC | ||||
stx _{1} | stx1-F | ACACTGGATGATCTCAGTGG | 35 cycles of 1 min at 95ï¿½C and 2 min at 65ï¿½C for the first 10 cycles, gradually decreasing to 60ï¿½C by cycle 15, and 1.5 min at 72ï¿½C | 614 | 15 |
stx1-R | CTGAATCCCCCTCCATTATG | ||||
stx _{2} | stx2-F | GGCACTGTCTGAAACTGCTCC | 35 cycles of 1 min at 95ï¿½C and 2 min at 65ï¿½C for the first 10 cycles, gradually decreasing to 60ï¿½C by cycle 15, and 1.5 min at 72ï¿½C | 255 | 14 |
stx2-R | TCGCCAGTTATCTGACATTCTG | ||||
eaeA | eaeA-F | GTGGCGAATACTGGCGAGACT | 35 cycles of 1 min at 95ï¿½C and 2 min at 65ï¿½C for the first 10 cycles, gradually decreasing to 60ï¿½C by cycle 15, and 1.5 min at 72ï¿½C | 890 | 15 |
eaeA-R | CCCCATTCTTTTTCACCGTCG | ||||
hlyA | hlyA-F | ACGATGTGGTTTATTCTGGA | 35 cycles of 1 min at 95ï¿½C and 2 min at 65ï¿½C for the first 10 cycles, gradually decreasing to 60ï¿½C by cycle 15, and 1.5 min at 72ï¿½C | 165 | 15 |
hlyA-R | CTTCACGTGACCATACATAT | ||||
espB | epsB-F | CGGGATCCCGTGAGATGGTCAC | 30 cycles of 1 min at 95ï¿½C, 1 min at 55ï¿½C, and 1 min at 72ï¿½C | 600 | 16 |
espB-R | CGCTCGAGGGTTGGACTTGACA | ||||
espB_{O157} | espB/O157-F | GATAATACTCAAGTAACGATGGTT | 30 cycles of 1 min at 95ï¿½C, 1 min at 59ï¿½C, and 1 min at 72ï¿½C | 920 | 16 |
espB/O157-R | CCCAGCTAAGCGACCCGATT | ||||
rfb_{O157} | PF8 | CGTGATGATGTTGAGTTG | 30 cycles of 1 min at 94ï¿½C, 1 min 53ï¿½C, and 1 min 72ï¿½C | 420 | 17 |
PR8 | AGATTGGTTGGCATTACTG | ||||
fliC H7 | 1806 | GCTGCAACGGTAAGTGAT | 30 cycles of 1 min at 94ï¿½C, 1 min 53ï¿½C, and 1 min 72ï¿½ | 948 | 17 |
1809 | GGCAGCAAGCGGGTTGGT |
↵a Cycling conditions and references apply to both primers in each primer pair.