Table 1

Expression ratios of selected proteins after cultivation of EDL933 in SIEM and SCEM under aerobic and microaerobic conditionsa

Growth conditions and proteinNameExpression ratiobFunction
SIEM aerobic
    Elongation factor TuTuf12.3*Protein biosynthesis
    Purine nucleoside phosphorylaseDeoD8.0Nucleotide biosynthesis
    F0F1 ATP synthase, subunit betaAtpD4.0Energy metabolism
    F0F1 ATP synthase, subunit deltaAtpH2.7Energy metabolism
    NADH dehydrogenase I, subunit FNuoF0.2Energy metabolism
    Malate dehydrogenaseMdh0.3General metabolism
    Chaperonin GroELGroEL14.2Chaperone
    Protein disaggregation chaperoneClpB5.7*Chaperone
    Phage shock proteinPspA4.3Stress-associated protein
    Glutamate decarboxylase isozymeGadB9,2*Glutamate-dependent acid resistance
    Glutamate decarboxylase isozymeGadA3.4Glutamate-dependent acid resistance
    S-RibosylhomocysteinaseYgaG0.2*Chemotaxis
    Hypothetical proteinZ11279.0Hypothetical protein O-island 43
    Putative phage inhibition, colicin resistance, and tellurite resistance proteinTerZ0.2Putative protein O-island 43
SIEM microaerobic
    Purine nucleoside phosphorylaseDeoD3.0Nucleotide biosynthesis
    Hydrogenase 1, large subunitHyaB5.7Energy metabolism
    Large subunit of hydrogenase 3HycE3.5Energy metabolism
    NADH dehydrogenase I, subunit FNuoF3.2Energy metabolism
    NADH dehydrogenase, subunit GNuoG2.7Energy metabolism
    Formate dehydrogenaseFdhF2.7Energy metabolism
    Hydrogenase 2, large subunitHybC2.0Energy metabolism
    Fumarase BFumB3.0General metabolism
    Malate dehydrogenaseMdh0.4General metabolism
    Glutamate decarboxylase isozymeGadA8.6Glutamate-dependent acid resistance
    FKBP-type peptidyl-prolyl cis-trans isomeraseFkpA4.9Stress-induced protein
    Curved DNA-binding proteinCbpA3.6Stress-induced protein
    Protein disaggregation chaperoneClpB2.7*Chaperone
    Chaperonin GroELGroEL2.2Chaperone
    Transcriptional regulatorBolA0.4Stress-induced protein
    Outer membrane proteinSlp0.1Stress-induced protein
    S-RibosylhomocysteinaseYgaG0.3Chemotaxis
    Putative phage inhibition, colicin resistance and tellurite resistance proteinTerB0.3Putative protein O-island 43
SCEM aerobic
    Purine nucleoside phosphorylaseDeoD0.3Nucleotide biosynthesis
    NADH dehydrogenase, subunit GNuoG4.0Energy metabolism
    F0F1 ATP synthase, subunit alphaAtpA3.2Energy metabolism
    F0F1 ATP synthase, subunit betaAtpD2.1Energy metabolism
    F0F1 ATP synthase, subunit deltaAtpH0.5Energy metabolism
    Glyceraldehyde-3-phosphate dehydrogenaseGapA6.4General metabolism
    Bifunctional acetaldehyde-CoA/alcohol dehydrogenaseAdhE3.0*General metabolism
    Pyruvate kinasePykF2.0*General metabolism
    PhosphomannomutaseManB7.5Cell structure
    S-RibosylhomocysteinaseYgaG0.3Motility
    FlagellinFliC0.1*Chemotaxis
    Putative exoprotein precursorEspP2.5Virulence determinant
    Cystine transporter subunitFliY0.5Amino acid transport
    Glutamine ABC transporter periplasmic proteinGlnH0.2Amino acid transport
    Hypothetical protein933Wp4240.1*Hypothetical protein phage protein
    Serine/threonine kinaseStk5.9Phage protein
    Hypothetical proteinZ03193.6Hypothetical protein phage protein
    Transcriptional regulatorRcsB3.3Colonic acid biosynthesis
    Hypothetical proteinZ23862.8Hypothetical protein phage protein
    Putative phage inhibition, colicin resistance, and tellurite resistance proteinTerB0.5Putative protein O-island 43
    Putative phage inhibition, colicin resistance, and tellurite resistance proteinTerD0.4Putative protein O-island 43
    Hypothetical proteinZ12130.3Hypothetical protein O-island 43
    Putative phage inhibition, colicin resistance, and tellurite resistance proteinTerE0.3Putative protein O-island 43
SCEM microaerobic
    GTP-binding proteinYihK4.9Protein biosynthesis
    Purine nucleoside phosphorylaseDeoD2.7Nucleotide biosynthesis
    Large subunit of hydrogenase 3HycE6.4*Energy metabolism
    Hydrogenase 1, large subunitHyaB5.9Energy metabolism
    Hydrogenase 1, small subunitHyaA3.6Energy metabolism
    Formate dehydrogenase-N, alpha subunitFdnG3.1Energy metabolism
    F0F1 ATP synthase, subunit betaAtpD2.6Energy metabolism
    Bifunctional acetaldehyde-CoA/alcohol dehydrogenaseAdhE3.4*General metabolism
    Pyruvate kinasePykF3.3*General metabolism
    Glucose-6-phosphate isomerasePgi2.6General metabolism
    PhosphomannomutaseManB6.3*Cell structure
    Transcriptional regulator BolABolA0.3Stress-associated protein
    FlagellinFliC0.1Motility
    Secreted proteinEspB3.1Virulence determinant
    Cystine transporter subunitFliY0.4Amino acid biosynthesis
    Glutamine ABC transporter, periplasmic proteinGlnH0.3Amino acid biosynthesis
    Putative phage inhibition, colicin resistance and tellurite resistance proteinTerZ0.3*Putative protein O-island 43
  • a For complete data, see Tables S1 to S4 in the supplemental material.

  • b An asterisk indicates that the protein was found in more than one spot.