Table 3

β-Galactosidase activity in bgaB reporter strains cultured in MM medium supplemented with sugar additivesa

StrainPromoterPossible inducerMean β-galactosidase activity (U/mg protein) ± SD with various sugar additives
Noned-Glucosed-Galactosed-Fructosed-Xylosel-Arabinosemyo-InositolMaltoseCellobioseMelibioseSucroseLactoseLBb
MK93Pgk704Maltose28 ± 131 ± 1323 ± 226 ± 23.3 ± 0.09.2 ± 0.625 ± 1330 ± 1030 ± 131 ± 327 ± 121 ± 215 ± 8
MK95PsigA3.1 ± 0.14.2 ± 0.32.2 ± 0.73.4 ± 0.50.8 ± 0.11.8 ± 0.12.3 ± 0.616 ± 12.3 ± 0.43.5 ± 0.53.2 ± 0.22.8 ± 0.24.4 ± 1.1
MK96None<0.1<0.1<0.1<0.1<0.1<0.1<0.1<0.1<0.1<0.1<0.1<0.1<0.1
MK111Pgk1894myo-Inositol32 ± 618 ± 519 ± 228 ± 31.2 ± 0.13.0 ± 0.5120 ± 3012 ± 126 ± 332 ± 916 ± 314 ± 10.4 ± 0.2
MK112Pgk1899myo-Inositol180 ± 30140 ± 7140 ± 10140 ± 2016 ± 140 ± 1440 ± 10130 ± 20200 ± 10160 ± 30160 ± 10130 ± 1041 ± 12
MK115Pgk2150d-Galactose2.1 ± 0.21.6 ± 0.319 ± 41.9 ± 0.20.4 ± 0.01.7 ± 0.61.4 ± 0.21.7 ± 0.21.2 ± 0.13.7 ± 0.31.5 ± 0.26.8 ± 0.21.2 ± 0.3
MK116Pgk1859Cellobiose0.1 ± 0.00.1 ± 0.00.1 ± 0.00.1 ± 0.00.1 ± 0.00.1 ± 0.00.1 ± 0.00.1 ± 0.00.4 ± 0.00.1 ± 0.00.1 ± 0.01.8 ± 0.20.1 ± 0.0
MK118Pgk1907l-Arabinose0.7 ± 0.10.5 ± 0.10.6 ± 0.10.5 ± 0.00.2 ± 0.00.4 ± 0.00.5 ± 0.10.6 ± 0.10.6 ± 0.10.6 ± 0.10.6 ± 0.00.6 ± 0.10.6 ± 0.1
  • a G. kaustophilus strains were cultured in medium indicated until the late logarithmic phase and analyzed for β-galactosidase activity. The values are means ± the SD from four independent experiments.

  • b LB medium.