Table 1

Summary of operating conditions for various MiSeq sequencing runs and machine-reported quality metricsa

Run[DNA] (pM)Cluster density (103/mm2)% PhiX% of bases ≥Q30bReported machine error (%)No. of 16S rRNA pairs (×106)
13040110.01,3133.863.71.3112.6
1304035.01,0943.270.50.8912.4
1304173.08396.274.60.9210.5
1304221.86908.080.10.659.0
  • a The four runs were performed using RTA v. 1.17.28 and MCS v. 2.2.0. Data for runs performed using previous versions of the software are provided in Tables S1 and S2 in the supplemental material.

  • b Percentage of bases with a quality score of at least 30.