Table 2

Summary of the error rates and number of observed OTUs for the sequencing runs described in Table 1

Region and runError rate (%) for:% reads remaining from basic (ΔQ = 0)Average no. of OTUsa
BasicΔQ = 6PreclusterMockbMockcSoilMouseHuman
V34
    1304012.140.370.2110.326.949.61,110.6175.1187.5
    1304031.300.260.1227.631.147.81,095.8158.2164.1
    1304171.120.240.1027.935.152.21,038.6ND142.6
    1304220.910.290.1747.541.454.31,053.0162.5145.1
V4
    1304011.080.060.0144.223.543.41,248.2136.1115.4
    1304030.670.050.0160.123.540.91,261.8133.9117.8
    1304170.400.050.0169.322.837.51,257.3135.5117.2
    1304220.280.050.0178.423.237.21,256.2132.8117.3
V45
    1304014.600.870.6413.5191.9271.71,462.8198.0312.9
    1304033.310.790.5632.3180.4246.11,519.7213.3324.0
    1304172.380.660.4336.5110.8158.3ND180.4242.1
    1304221.670.580.3656.498.0131.61,403.3186.3227.2
  • a The average number of OTUs is based on rarefaction of each sample to 5,000 sequences per sample; cells labeled ND reflect samples that did not have at least one replicate with more than 5,000 sequences.

  • b Number of OTUs in the mock community when all chimeras were removed; in the absence of chimeras and sequencing errors, there should be 20 OTUs for all three regions.

  • c Number of OTUs in the mock community when chimeras were removed using UCHIME.