Table 1

Bacterial strains and plasmids

Strain or plasmidRelevant traitsaSource or reference(s)
Strains
    Pseudomonas spp.
        P. aeruginosa PAO175
        P. brassicacearum Q8r1-96Isolated from wheat rhizosphere in Washington, USA; biological control of take-all of wheat; plasmidlessD. Weller (76)
        P. chlororaphis O6Isolated from soil; biological control of multiple biotic and abiotic stresses of plants; plasmidlessA. Anderson (77)
        P. fluorescens A506Isolated from pear phyllosphere in California, USA; registered commercial biological control agent for fire blight; single plasmid, pA506; Rf Sm10
        P. fluorescens A506 pA506::gfp-km-gmSingle insertion of gfp-km-gm cassette from pTGN in pA506 in PflA506_p0022; GFP+; Gm Km Rf SmThis study
        P. fluorescens A506-ΔpA506Plasmidless derivative of A506; Rf SmThis study
        P. fluorescens SS101Isolated from wheat rhizosphere in The Netherlands; biological control of diseases caused by oomycetes, i.e., Pythium and Phytophthora species; plasmidlessJ. Raaijmakers (78)
    Enterobacteriaceae
        Escherichia coli K-12ATCC 15153, SmAmerican Type Culture Collection
        E. coli S-17 (λpir)pro thi hsdR3M, RP4-2-Tc:Mu-Km:Tn7 λpir; Sm26
        Pantoea vagans C9-1Isolated from apple phyllosphere in Michigan USA; registered biological control agent for fire blight; three large plasmids (pPag1, pPag2, pPag3)79, 80
Plasmids
    pCR2.1-TOPOpCR2.1-TOPO cloning vector; KmInvitrogen
    pTGNpBSL202 with mini-Tn5 gfp-km-gm; transposon including a promoterless gfp-Km and constitutively expressed Gm26
    pA506::gfp-km-gmSingle insertion of gfp-km-gm cassette of pTGN in PflA506_p0022This study
  • a Gm, Km, Rf, and Sm, resistance to gentamicin, kanamycin, rifampin, and streptomycin, respectively.