Table 2

Substrate-specific biomass yield (YX/S), biomass-specific glucose consumption rate (qS), and substrate-specific l-alanine and l-valine yields (YP/S) of C. glutamicum l-valine producer strains cultivated in CGXII medium with 4% (wt/vol) glucose in shake flasksa

StrainYX/S(g CDW/mmol glucose)qS [g glucose/(g CDW × h)]YP/S (mol/mol glucose)
l-Alaninel-Valine
C. glutamicum WT (pJC4 ilvBNCE)0.066 ± 0.0120.80 ± 0.150.02 ± 0.010.11 ± 0.04
C. glutamicum aceE L1 (pJC4 ilvBNCE)0.024 ± 0.0041.00 ± 0.170.06 ± 0.010.21 ± 0.01
C. glutamicum aceE A23 (pJC4 ilvBNCE)0.027 ± 0.0030.91 ± 0.100.07 ± 0.010.25 ± 0.01
C. glutamicum aceE A25 (pJC4 ilvBNCE)0.040 ± 0.0030.80 ± 0.060.04 ± 0.010.35 ± 0.02
C. glutamicum aceE A16 (pJC4 ilvBNCE)0.042 ± 0.0020.77 ± 0.040.02 ± 0.010.37 ± 0.01
C. glutamicum aceE A16 Δpqo (pJC4 ilvBNCE)0.042 ± 0.0030.72 ± 0.050.02 ± 0.010.40 ± 0.03
C. glutamicum aceE A16 Δpqo Δppc (pJC4 ilvBNCE)0.039 ± 0.0050.80 ± 0.110.02 ± 0.010.42 ± 0.02
  • a Values represent the arithmetic mean ± standard deviation from at least three independent experiments.