Table 1

Predicted sizes of PCR products and the RFLP fragments of various cry-type genes

Primer pair and genePredicted size (bp) of PCR product(s)
Before digestionAfter digestion with HinfI
CRY1P1 and CRY1PR
    cry1Aa1,921650, 561, 334, 330, 68
    cry1Ab1,924724, 346, 334, 330, 212
    cry1Ac1,927392, 346, 335, 334, 330, 212
    cry1Ae1,924664, 494, 346, 230, 212
    cry1Af1,915724, 346, 334, 321, 212
    cry1Ag1,921576, 561, 334, 330, 74, 68
    cry1Ai1,924561, 335, 334, 330, 318, 68
    cry1La1,894762, 721, 240, 150, 43
CRY1P2 and CRY1PR
    cry1Ba2,008689, 322, 244, 168, 132, 118, 112, 72, 53, 48, 48, 24
    cry1Bb2,0231,114, 221, 149, 144, 138, 137, 48, 48, 18, 15, 13
    cry1Bc2,0231,114, 221, 149, 144, 138, 137, 48, 48, 18, 15, 13
    cry1Bd2,0291,086, 658, 137, 48, 48, 46, 15, 13
    cry1Be2,0171,003, 358, 215, 147, 108, 53, 48, 48, 26, 18, 15
    cry1Bf2,020801, 358, 276, 215, 132, 53, 48, 48, 46, 26, 24, 15
    cry1Bg2,041689, 358, 339, 215, 132, 112, 67, 53, 48, 26, 24
    cry1Bh2,023822, 627, 176, 147, 108, 48, 48, 31, 18, 15, 5
    cry1Ka2,0111,001, 505, 266, 106, 92, 48, 15
CRY1P3 and CRY1PR
    cry1Ad1,921718, 643, 561, 21
    cry1Ah1,924643, 389, 346, 335, 212, 21
    cry1Ca1,948702, 645, 517, 106
    cry1Cb1,909521, 517, 302, 243, 181, 116, 51
    cry1Da1,876860, 697, 276, 65
    cry1Db1,8761,136, 585, 112, 65
    cry1Dc1,900805, 712, 192, 127, 65, 21
    cry1Ea1,903727, 709, 468, 21
    cry1Eb1,897475, 296, 294, 287, 270, 128, 116, 53
    cry1Fa1,903677, 476, 293, 140, 134, 114, 91
    cry1Fb1,900489, 293, 283, 188, 153, 140, 114, 106, 91, 65
    cry1Ga1,879552, 429, 403, 252, 131, 116, 18
    cry1Gb1,888934, 350, 262, 258, 106
    cry1Ha1,897825, 715, 192, 127, 39, 21
    cry1Hb1,888828, 354, 225, 192, 130, 97, 48, 36
    cry1Ja1,885516, 364, 349, 186, 168, 153, 106, 65
    cry1Jb1,888702, 444, 322, 267, 73, 53, 49
    cry1Jd1,888517, 448, 364, 274, 248, 59
CRY1P4 and CRY1PR
    cry1Ia1,917786, 315, 303, 145, 130, 111, 53, 48, 31, 17
    cry1Ib1,917497, 315, 256, 211, 176, 147, 108, 92, 53, 48, 31, 5
    cry1Ic1,917571, 497, 211, 181, 147, 108, 92, 53, 48, 31
    cry1Id1,917624, 564, 303, 222, 147, 48, 31
    cry1Ie1,917558, 315, 256, 228, 211, 130, 92, 53, 48, 31, 17
    cry1Ma1,9081,270, 303, 130, 100, 95, 32