Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristics/sequenceSource or reference
Strains
    V. parahaemolyticus
        1137Wild type, serotype O3:K6, KP+; clinical isolateThis study
        Δ24681137 ΔdacBThis study
        Δ2468C1137 ΔdacB containing pSCB01 dacBThis study
        Δ2468V1137 ΔdacB containing pSCB01This study
    E. coli
        XL1-BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]Stratagene
        SM10λpirthi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λ pirR6K; Kmr46
Plasmids
    pGEM-T EasyCloning vector; AprPromega
    pDS132R6K ori mobRP4 sacB Cmr47
    pBR328Vector derived from pBR325 and pMB1; Apr Cmr TcrRoche
    pSCB01Derived from pBR328 and pDS132; mobRP4 Apr Cmr TcrThis study
    pGEM-T dacBpGEM-T containing dacBThis study
    pDS132ΔdacBpDS132 with ΔdacBThis study
    pSCB01 dacBpSCB01 containing dacBThis study