Table 1

Metabolite concentrations in perchloric extracts from cell suspensions of B. animalis subsp. lactis BB-12a

Cell and substrateMean ± SD metabolite concn (mM)% carbon recovery by NMR
Substrate isotopomerAcetateLactateEthanol[1-13C] formate by total NMRGlc-6-PGal-1-P
[1,2-13C]acetate[1-13C]acetate[2-13C]acetateTotal acetateTotal by NMR[2,3-13C]lactate[2-13C]lactate[3-13C]lactateTotal lactateTotal by NMR[1,2-13C]EtOH[1-13C]EtOH[2-13C]EtOHTotal EtOHTotal by NMR
Glc (20 mM)-grown cells
    [1-13C]Glc0.66 ± 0.160.08 ± 0.1116.80 ± 0.4222.45 ± 0.6439.33 ± 1.170.889.45 ± 0.4910.33 ± 0.490.99 ± 0.041.95 ± 0.072.95 ± 0.047.45 ± 0.210.10 ± 0.04106.45 ± 2.90
    [2-13C]Glc18.15 ± 0.490.21 ± 0.2121.5 ± 0.4239.86 ± 0.280.17 ± 0.030.04 ± 0.033.55 ± 0.073.74 ± 0.130.93 ± 0.801.90 ± 1.132.83 ± 1.9314.10 ± 0.7192.25 ± 2.76
    [3-13C]Glc8.85 ± 0.780.55 ± 0.070.55 ± 0.0727.4 ± 0.2837.3 ± 0.420.211.85 ± 0.0712.05 ± 0.070.70 ± 0.141.65 ± 0.212.35 ± 0.356.70 ± 0.14101.05 ± 0.21
Lac (15 mM)-grown cells
    [1-13CGlc]Lac0.44 ± 0.6212.2 ± 0.4240.45 ± 1.3452.65 ± 1.770.13 ± 0.0119.70 ± 0.4219.83 ± 0.410.07 ± 0.031.75 ± 0.071.82 ± 0.106.30 ± 0.28100.15 ± 7.00
    [1-13CGal]Lac1.05 ± 0.1310.85 ± 0.6432.45 ± 0.2143.30 ± 0.850.10 ± 0.0318.55 ± 0.0718.65 ± 0.100.031.40 ± 0.281.43 ± 0.282.40 ± 0.570.05 ± 0.0192.80 ± 2.83
    [1-13C]Glc0.35 ± 0.2911.25 ± 0.6418.00 ± 0.8529.25 ± 1.480.24 ± 0.035.05 ± 0.355.29 ± 0.380.54 ± 0.252.10 ± 0.142.64 ± 0.116.65 ± 0.640.18 ± 0.1599.15 ± 6.01
  • a B. animalis subsp. lactis BB-12 was grown in MRSfc plus Glc after one pulse of 20 mM [1-13C]Glc, [2-13C]Glc, or [3-13C]Glc or grown in MRSfc plus Lac after one pulse of 15 mM [1-13CGlc]Lac, [1-13CGal]Lac, or [1-13C]Glc, as quantified by 13C NMR and 1H NMR. Values represent means ± standard deviations from duplicate assays. EtOH, ethanol; Glc-6-P, Glc 6-phosphate; Gal-1-P, α-galactose 1-phosphate.