Recipient strain | Plasmid | Avg acquisition ± SD (%)^{a} | |
---|---|---|---|

Km^{r} (Km^{r} Ap^{r})^{b} | Ap^{r} (Ap^{r} Km^{r})^{c} | ||

P. stutzeri AN10 | pGP704Km | (6.9 ± 0.9) × 10^{−7} (82) | (6.5 ± 1.2) × 10^{−7} (79) |

pJOC22Km | (3.4 ± 0.7) × 10^{−4} (0) | (7.4 ± 0.4) × 10^{−7} (82) | |

P. stutzeri AN11 | pGP704Km | (6.1 ± 1.5) × 10^{−8} (74) | (6.2 ± 2.3) × 10^{−8} (80) |

pJOC22Km | (5.8 ± 0.9) × 10^{−8} (78) | (6.5 ± 1.1) × 10^{−8} (77) |

↵a E. coli S17-1λ

was used as a donor strain. The values are the averages of three independent mating experiments._{pir}↵b Frequencies of recipient cells that received the Km

^{r}determinant. Frequencies were calculated by dividing the number of Km^{r}transconjugants obtained by the number of recipient cells counted immediately after conjugation. Transconjugants were selected on MBM plates supplemented with succinate and kanamycin. Recipient strains were counted on MBM plates supplemented with succinate. The percentage of Km^{r}transconjugants that also acquired the Ap^{r}determinant of the vector is indicated after each value in parentheses.↵c Frequencies of recipient cells that received the Ap

^{r}determinant. Frequencies were calculated by dividing the number of Ap^{r}transconjugants obtained by the number of recipient cells counted immediately after conjugation. Transconjugants were selected on MBM plates supplemented with succinate and ampicillin. Recipient strains were counted on MBM plates supplemented with succinate. The percentage of Ap^{r}transconjugants that also acquired the Km^{r}determinant of the vector is indicated after each value in parentheses.