Table 2

Ten most abundant Lactobacillus OTUs (clustered at ≥97% sequence identity) associated with three ant and three bee species (bee species are binned as halictids)a

HostSource of readNo. of readsClosest phylogenetic relativeBest BLAST hitSequence identity (%)
Solenopsis Brood geminata TX5,993Undescribed L. sakei 92
Brood geminata TW2,118 L. plantarum L. plantarum 99
Brood invicta TX935 L. curvatus L. curvatus 99
Brood invicta TX756 L. brevis L. brevis 99
Pooled invicta TW661 L. plantarum L. plantarum 99
Pooled invicta TW324 L. plantarum L. plantarum 99
Pooled geminata TW261 L. paracasei L. casei 99
Brood geminata TW167 L. kimchicus L. odoratitofui 99
Brood geminata TW156Undescribed L. sakei 93
Brood geminata TW116 L. vaccinostercus L. vaccinostercus 99
Mycocepurus smithii Garden14,152 L. johnsonii L. johnsonii 99
Worker3,748 L. crispatus L. crispatus 99
Garden2,794 L. salivarius L. salivarius 97
Garden1,059 L. aviarius L. aviarius 99
Garden806 L. crispatus L. crispatus 98
Worker598 L. salivarius L. salivarius 99
Garden586 L. reuteri L. reuteri 99
Worker431 L. salivarius L. salivarius 98
Worker420 L. aviarius/L. johnsonii L. aviarius 90
Garden408 L. johnsonii L. johnsonii 95
HalictidsLarva20,669 L. kunkeei/L. ozensis L. kunkeei 93
Pollen15,761 L. fructivorans L. fructivorans 91
Larva3,923 L. kunkeei/L. ozensis L. kunkeei 93
Pollen3,631 L. kunkeei/L. ozensis L. kunkeei 94
Pollen1,991 L. kunkeei/L. ozensis L. kunkeei 93
Pollen1,557 L. fructivorans L. fructivorans 90
Larva1,455 L. kunkeei/L. ozensis L. kunkeei 93
Pollen1,285 L. kunkeei/L. ozensis L. kunkeei 94
Frass1,102 L. paracasei L. casei 99
Pollen397 L. kunkeei/L. ozensis L. kunkeei 94
  • a The source of read indicates the types of samples from which the OTUs were isolated (TX, Texas; TW, Taiwan; pooled, workers and brood from five colonies). The number of reads represents the number of reads that cluster into each OTU. The closest phylogenetic relative is the closest Lactobacillus species in our phylogenetic reconstruction with which a particular read clustered (Fig. 2). The best BLAST hit is the top hit to a named Lactobacillus species from a BLAST search of NCBI's entire nucleotide collection. The sequence identity is the percent sequence identity of the query to the best BLAST hit. Several OTUs shared top BLAST hits to the same Lactobacillus species but shared <97% sequence identity to each other.