Table 3

Number of BF-1 cells in the feces of 12 volunteers who ingested a fermented milk product containing BF-1 for 28 days

SubjectNo. of BF-1 cells (log cells/g feces or log CFU/g feces [wet weight])
Quantified before ingestionaQuantified after ingestion
qPCRbCultureqPCRCulture
Without PMAWith PMAWithout PMAWith PMA
a<5.3<5.3<26.85.54.9
b<5.3<5.3<28.16.23.3
c<5.3<5.3<28.06.55.1
d<5.3<5.3<28.06.66.6
e<5.3<5.3<28.25.94.8
f<5.3<5.3<28.56.65.3
g<5.3<5.3<27.86.46.1
h<5.3<5.3<28.25.72.8
i<5.3<5.3<26.76.02.0
j<5.3<5.3<27.65.93.7
k<5.3<5.3<27.76.96.5
l<5.3<5.3<25.95.82.9
  • a The lower limits of detection of qPCR and the culture method were 105.3 cells/g feces (wet weight) and 102.0 CFU/g feces, respectively.

  • b PMA, propidium monoazide; qPCR, real-time quantitative PCR.