Table 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaSource or reference
Bacterial strains
    RN4220Highly transformable S. aureus 14
    JE2USA300 CA-MRSA strain LAC without plasmids 8
    NE1260JE2 pckA::ϕΝΣ 8
    NE1260CJE2 pckA::ecfpThis study
    NE1260FJE2 pckA::fp650This study
    NE1260GJE2 pckA::sgfpThis study
    NE1260RJE2 pckA::rfpThis study
    NE1260RRJE2 pckA::rfp with rfp in opposite directionThis study
    NE1260YJE2 pckA::eyfpThis study
    NE1354JE2 hla::ϕΝΣ 8
    NE1354KNE1354 with aphA-3 (Kanr) replacementThis study
    NE1354TNE1354 with tet(M) (Tetr) replacementThis study
    NE1354TnTNE1354 with truncated TnThis study
    NE1354SNE1354 with aad9 (Spcr) replacementThis study
Plasmids
    pBFP-FpTnT with ecfp in forward orientationThis study
    pBFP-RpTnT with ecfp in reverse orientationThis study
    pCL10pET194ts oriV, Chlr Ampr, pBR322 oriV 22
    pCN34Source of apha-3 21
    pCN36Source of tet(M) 21
    pCN55Source of aad9 21
    pFP650-FpTnT with fp650 in forward orientationThis study
    pFP650-RpTnT with fp650 in reverse orientationThis study
    pGFP-FpTnT with sgfp in forward orientationThis study
    pGFP-RpTnT with sgfp in reverse orientationThis study
    pJB33pCL10 with Pxyl/tetO-secY570This study
    pJB38pJB33 with XhoI site removedThis study
    pJB68Source of sgfpLab stock
    pKANpTnT with aphA-3This study
    KOR1Source of Pxyl/tetO-secY570 23
    pRFP-FpTnT with rfp in forward orientationThis study
    pRFP-RpTnT with rfp in reverse orientationThis study
    pSPCpTnT with aad9This study
    pTETpTnT with tet(M)This study
    pTnTpJB38 with homologous DNA to bursa aurealisThis study
    pYFP-FpTnT with eyfp in forward orientationThis study
    pYFP-RpTnT with eyfp in reverse orientationThis study
  • a Antibiotic resistance abbreviations: Ampr, ampicillin resistance (bla); Chlr, chloramphenicol resistance (cat); Kanr, kanamycin resistance (aphA-3); Tetr, tetracycline resistance [tet(M)]; Spcr, spectinomycin resistance (aad9). rfp, ecfp, eyfp, sgfp, and fp650 encode DsRed.T3(DNT), ECFP, EYFP, superfolder GFP, and eqFP650, respectively.