Table 2

Amplicon length distribution of primer hits categorized by ITS locus

ITS locusForward primerReverse primerAmplicon length distribution (mean ± SD)
AscomycotaBasidiomycotaNon-Dikaryaa
ITS1BITSB58S3183.6 ± 46.8219.8 ± 56.9215.0 ± 95.9
ITS1ITS2218.1 ± 54.4253.3 ± 60.0201.0 ± 71.8
NSI158A2357.2 ± 128.9386.5 ± 105.7269.1 ± 24.1
ITS1F_KYO1ITS2_KYO1275.3 ± 103.2285.3 ± 50.1200.3 ± 54.2
ITS1F_KYO2ITS2_KYO2270.6 ± 90.5284.5 ± 42.1216.4 ± 94.4
ITS258A1NLB4478.8 ± 23.9528.1 ± 30.3
58A2NLB4476.8 ± 23.9525.9 ± 30.4
ITS3F_KYO1ITS4_KYO1310.5 ± 29.9362.2 ± 35.3376.0 ± 57.4
fITS7fITS4258.5 ± 27.3309.8 ± 35.6312.7 ± 47.2
gITS7fITS4259.9 ± 22.5307.6 ± 34.7312.9 ± 47.4
fITS9fITS4324.4 ± 11.7354.6 ± 32.9
Whole ITSBITSITS4535.8 ± 81.8618.0 ± 72.6573.0 ± 132.4
ITS1ITS4547.2 ± 93.0624.8 ± 79.7582.4 ± 132.0
NSI1NLB41,066.6 ± 313.9927.3 ± 182.6
ITS1F_KYO1ITS4_KYO1612.4 ± 131.1664.9 ± 57.8589.8 ± 155.6
  • a Missing values are due to noncoverage of non-Dikarya by that primer.