Table 3

Xenobiotic substrate specificity and oxidation activity of CYP63A2

Xenobiotic (class and compound)Structural variableCYP63A2 activityMean oxidation (%) (±SD)a
No. of ringsAlkyl chain length (mixture of p isomers)No. of carbon atoms
Aromatic/polycyclic aromatic hydrocarbons
    Benzo(ghi)perylene6Yes18.5 (±3.7)
    Benzo(a)pyrene5Yes35.7 (±2.3)
    Fluoranthene4Yes24.5 (±4.7)
    Pyrene4Yes21.0 (±3.6)
    tNPYes43.3 (±3.4)
    4-n-NonylphenolC9Yes88.2 (±4.5)
    4-n-OctylphenolC8Yes97.1 (±3.6)
    4-n-HeptylphenolC7Yes90.2 (±5.0)
    4-n-PentylphenolC5Yes87.5 (±3.6)
    4-n-ButylphenolC4Yes85.1 (±3.7)
    4-n-PropylphenolC3Yes60.1 (±5.7)
Aliphatic hydrocarbons: n-alkanes9–12, 15–19Yes9.1–31.1b
  • a Values are for three biological replicates. Each culture was spiked with an individual PAH or an alkylphenol, and the oxidation activity was assessed at 24 h of incubation.

  • b For details on oxidation of individual alkanes, see Table 5.

  • c ND, not detected.