TABLE 2

Numbers of translated nifH sequences and OTUs, diversity, and coverage at different amino acid sequence similarity levels in ambient and elevated CO2 plots across sitesa

OTU similarity (%)SiteNo. of sequencesNo. of OTUs1/DCoverage
AmbElvAmbElvAmbElvAmbElv
99MDE38340817018540.953.90.710.71
NCD121121536420.035.5b0.790.65
TNO116171606641.120.50.690.78
WIR13315936405.335.460.830.88
95MDE3834089510222.827.50.870.88
NCD12112117296.024.920.960.84
TNO11617118276.784.230.950.92
WIR13315919194.574.050.950.97
90MDE38340847486.129.290.940.96
NCD1211219143.702.970.980.93
TNO11617111163.383.590.970.96
WIR13315910103.003.100.990.99
  • a Amb, ambient; Elv, elevated. Temperate brackish tidal marsh (MDE), n = 5; warm temperate pine plantation (NCD), n = 3; warm temperate deciduous plantation (TNO), n = 2; cool temperate deciduous plantation (WIR), n = 3. See Materials and Methods for detailed site and soil information.

  • b Significant effect of elevated CO2 (P < 0.05).