TABLE 1

Strains and plasmids used in this study

Strain or plasmidRelevant genotype and descriptionReference
E. coli
    DH5αλ ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA141
A. fabrum
    C58Wild type20
    C58ΔSpG8-1bC58 with Atu1410 to Atu1423 genes deleted; Neor Kanr23
    C58ΔAtu1415C58 with Atu1415 gene deletedThis study
    C58ΔAtu1416C58 with Atu1416 gene deletedThis study
    C58ΔAtu1420C58 with Atu1420 gene deleted; Neor KanrThis study
Plasmids
    pLARF1Broad-host-range cosmid vector; oriVRK2 oriTRK2; Tetr42
    pLAFR1::Atu1415-Atu1423Atu1415 to Atu1440 genes cloned in pLAFR1This study
    pJQ200SKSuicide vector; P15A sacB; Gmr43