TABLE 3

ka and catalytic efficiency (kcat/Km) of XynA and XynA-Tr toward xylooligosaccharides of different lengths

Hydrolysis productka (M−1 [103])kcat/Km (mM−1 min−1)
XynAXynA-TrXynAXynA-Tr
Xylohexaose274 ± 21.11.45 ± 0.13831.4 ± 2.01.24 ± 0.03
Xylopentaose241 ± 14.43.05 ± 0.08162.2 ± 2.00.60 ± 0.07
Xylotetraose134 ± 36.61.22 ± 0.03128.2 ± 0.51.12 ± 0.10
Xylotriose30.8 ± 4.10.46 ± 0.03135.6 ± 1.3NDa
  • a ND, not detected.