TABLE 1

Richness and diversity of phyllosphere microbiota based on pyrosequencing of bacterial (16S rRNA gene) and fungal (ITS fragment) communities collected from grapevine leaves

Leaf community and sampleaIdentified OTUbGood's coverageEstimated OTU richnessH′
Chao1ACE
Bacteria
    UD-UNT10696.991954.30
    UD-PEN7897.087924.23
    UD-AZ785198.370564.06
    SM1-UNT20194.11731866.01
    SM1-PEN21593.02022026.17
    SM1-AZ7820195.11581625.96
    SM2-UNT11494.81591513.19
    SM2-PEN8796.91321392.16
    SM2-AZ784996.5991173.05
Fungi
    UD-UNT11099.7102993.42
    UD-PEN12399.71271224.51
    UD-AZ7812199.71211163.54
    SM1-UNT12499.71401212.46
    SM1-PEN14299.61391402.67
    SM1-AZ7812599.81171163.02
    SM2-UNT13599.71451435.09
    SM2-PEN11099.71231274.62
    SM2-AZ7811799.81121134.75
  • a Leaf samples were collected from untreated plants (UNT) or plants treated with penconazole (PEN) or with Lysobacter capsici AZ78 (AZ78) grown in Udine (UD), San Michele all'Adige (SM1), and San Michele all'Adige protected from rain (SM2). Data of three replicates for each sample (reported in Table S2 in the supplemental material) were merged.

  • b Number of operational taxonomic units (OTU) identified with the UCLUST algorithm (41) at 97% sequence similarity.