Recovery of DNA in a 5-kb ladder recovered after filtration through AAO (0.02-μm Anotop 25) filters

Sample% recoverya
Filtrate64 (12)
Wash 110 (2)
Wash 22 (0.5)
Wash 30.6 (0.3)
Extract7 (2)
Total84 (12)
  • a Percentages of DNA in the filtrate and washes represent material passing through the filters, and that in the extract represents material recovered from the filters using a back-flush protocol. Values are means (standard deviations) from triplicate assays.