TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
P. fluorescens
    2P24Wild type; Apr64
    PM121gidA::Tn5 in strain 2P24; Apr KmrThis study
    PM701gidA gene in-frame deletion in strain 2P24; AprThis study
    PM702trmE gene in-frame deletion in strain 2P24; AprThis study
    PM703trmE gene in-frame deletion in mutant PM701; AprThis study
    PM915phlA gene in-frame deletion in strain 2P24; AprThis study
    PM917gidA gene in-frame deletion in mutant PM915; AprThis study
    PM918trmE gene in-frame deletion in mutant PM915; AprThis study
    PM919phlD gene in-frame deletion in mutant PM915; AprThis study
    PM920phlD gene in-frame deletion in strain 2P24; AprThis study
    PM1082P24 with a VSV-G epitope sequence at the C terminus of PhlA; AprThis study
    PM109PM701 with a VSV-G epitope sequence at the C terminus of PhlA; AprThis study
    PM110PM702 with a VSV-G epitope sequence at the C terminus of PhlA; AprThis study
    PM1112P24 with a VSV-G epitope sequence at the C terminus of PhlD; AprThis study
    PM112PM701 with a VSV-G epitope sequence at the C terminus of PhlD; AprThis study
    PM113PM702 with a VSV-G epitope sequence at the C terminus of PhlD; AprThis study
    PM114PM703 with a VSV-G epitope sequence at the C terminus of PhlD; AprThis study
    PM1152P24 with a VSV-G epitope sequence at the C terminus of RsmA; AprThis study
    PM116PM701 with a VSV-G epitope sequence at the C terminus of RsmA; AprThis study
    PM117PM702 with a VSV-G epitope sequence at the C terminus of RsmA; AprThis study
    PM1182P24 with a VSV-G epitope sequence at the C terminus of RsmE; AprThis study
    PM119PM701 with a VSV-G epitope sequence at the C terminus of RsmE; AprThis study
    PM120PM702 with a VSV-G epitope sequence at the C terminus of RsmE; AprThis study
E. coli
    DH5αλ ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA165
Fungus
    R. solaniPlant pathogen that causes cotton damping offLab stock
Plasmids
    pUTKmDelivery plasmid for Tn5; R6K replicon; Apr Kmr47
    pRK600Helper plasmid for triparental mating; Cmr66
    pBluescript II SK(+)Cloning vector; AprStratagene
    pBSKmSuicide plasmid for Pseudomonas spp., used for homologous recombination; derivative of pBluescript II SK(+); KmrThis study
    pBSKΔgidApBSKm carrying a deleted gidA gene; KmrThis study
    pBSKΔtrmEpBSKm carrying a deleted trmE gene; KmrThis study
    pBSKΔphlApBSKm carrying a deleted phlA gene; KmrThis study
    pBSKΔphlDpBSKm carrying a deleted phlD gene; KmrThis study
    pBBR1MCS-5pBBR1MCS-5 containing pBluescript II KS-lacZα; Gmr48
    pBBR5-gidApBBR1MCS-5 containing gidA gene; GmrThis study
    pBBR5-trmEpBBR1MCS-5 containing trmE gene; GmrThis study
    pBBR5-phlDpBBR1MCS-5 containing phlD gene; GmrThis study
    pBBR5-phlDVpBBR1MCS-5 containing phlD gene with a VSV-G epitope sequence at its C terminus; GmrThis study
    pRG970GmCloning vector containing promoterless lacZYA for construction of transcriptional fusion; Gmr32
    pRG970KmCloning vector containing promoterless lacZYA for construction of transcriptional fusion; Kmr67
    p970Km-phlAppRG970Km containing a phlA-lacZ transcriptional fusion; Kmr32
    p970Km-rsmXppRG970Km containing an rsmX-lacZ transcriptional fusion; Kmr33
    p970Gm-rsmYppRG970Gm containing an rsmY-lacZ transcriptional fusion; Gmr33
    p970Km-rsmZppRG970Km containing an rsmZ-lacZ transcriptional fusion; Kmr33
    p970Km-rsmAppRG970Km containing an rsmA-lacZ transcriptional fusion; Kmr33
    p970Gm-rsmEppRG970Gm containing an rsmE-lacZ transcriptional fusion; Gmr33
    pBSKPhlA-VSVpBSKm with a VSV-G epitope sequence at the C terminus of PhlA; KmrThis study
    pBSKPhlD-VSVpBSKm with a VSV-G epitope sequence at the C terminus of PhlD; KmrThis study
    pBSKRsmA-VSVpBSKm with a VSV-G epitope sequence at the C terminus of RsmA; KmrThis study
    pBSKRsmE-VSVpBSKm with a VSV-G epitope sequence at the C terminus of RsmE; KmrThis study
  • a Apr, Kmr, Gmr, and Cmr indicate resistance to ampicillin, kanamycin, gentamicin, and chloramphenicol, respectively.