TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
    Comamonas testosteroni
        CNB-1Wild type26
        CNB-1Δ0097bclA fragment of 0097bcl encoding amino acids 152 to 436 was deletedThis study
        CNB-1Δ0097bcl/pBBR1MCS3-bclComplementation for CNB-1Δ0097bclThis study
        CNB-1Δ0394bclA fragment of 0394bcl DNA was deletedThis study
        CNB-1Δ0097_0394bclCarries mutations of 0097bcl and 0394bclThis study
        CNB-1ΔboxABDNA fragment encoding BoxAB was deletedThis study
        CNB-1ΔboxAB/pBBR1MCS3-boxBAComplementation for CNB-1ΔboxABThis study
        CNB-1ΔboxCA fragment of boxC encoding amino acids 15 to 472 was deletedThis study
        CNB-1ΔboxC/pBBR1MCS3-boxCComplementation for CNB-1ΔboxCThis study
        CNB-1ΔgenAA fragment of genA encoding amino acids 1 to 332 was deletedThis study
        CNB-1ΔgenA_boxCCarries mutations of genA and boxCThis study
    E. coli
        DH5αϕ80 lacZ ΔM15 endA1 recA1 hsdR17(rK mK) supE44 ΔlacU16931
        BL21(DE3)F ompT hsdSB(rB mB) gal dcm λDE3 (harboring gene 1 of the RNA polymerase from the phage T7 under the control of the PlacUV5 promoter)31
        Rosetta(DE3)F ompT hsdSB(rB mB) gal dcm (DE3) pRARE (Camr)Novagen
Plasmids
    pK18mobsacBMobilizable vector; allows selection of double crossover in CNB-134
    pK18mobsacB0097bclConstruction of CNB-1Δ0097bcl and CNB-1Δ0097_0394bclThis study
    pK18mobsacB0394bclConstruction of CNB-1Δ0394bclThis study
    pK18mobsacBboxABConstruction of CNB-1ΔboxABThis study
    pK18mobsacBboxCConstruction of CNB-1ΔboxC and CNB-1ΔgenA_boxCThis study
    pK18mobsacBgenAConstruction of CNB-1ΔgenAThis study
    pBBR1MCS3Tcr, lacPOZ′ broad-host-range vector with R-type conjugative origin54
    pBBR1MCS3-bclCarries 0097bcl to generate complementation for 0097bclThis study
    pBBR1MCS3-boxBACarries boxBA to generate complementation for boxABThis study
    pBBR1MCS3-boxCCarries boxC to generate complementation for boxCThis study
    pBBR1MCS2-PgenA::eGFPpBBR1MCS2 derivative for fusion of PgenA and eGFPThis study
    pET28aExpression vectorNovagen
    pET28a-bclpET28a derivative for expression of 0097bclThis study
    pET28a-boxApET28a derivative for expression of boxAThis study
    pET28a-boxBpET28a derivative for expression of boxBThis study
    pET28a-boxCpET28a derivative for expression of boxCThis study
    pET28a-boxDpET28a derivative for expression of boxDThis study
    pET28a-genApET28a derivative for expression of genAThis study
    pET28a-genBpET28a derivative for expression of genBThis study
    pET28a-genCpET28a derivative for expression of genCThis study
    pET28a-genRpET28a derivative for expression of genRThis study