TABLE 2

Proteins related to aromatic compound degradationa

No.ORFProtein name; putative functionScorebMatchcCoverage (%)dpI/MWf
TheoreticaleExperimental
B6CtCNB1_2779GenB; fumarylpyruvate hydroxylase7712585.31/265.47/27
B13CtCNB1_0066BoxB; benzoyl-CoA oxygenase component B13318435.63/545.83/55
B14CtCNB1_0065BoxA; benzoyl-CoA oxygenase; reductase12029455.66/475.94/55
B15CtCNB1_0097BCL; benzoate-CoA ligase family6915315.90/576.10/59
B17CtCNB1_0097BCL; benzoate-CoA ligase family6115315.90/576.30/59
B19CtCNB1_2778GenA; gentisate 1,2-dioxygenase5411346.10/426.40/47
B22CtCNB1_0093LivG; ABC-type branched-chain amino acid transport systems, ATPase component5213485.99/286.51/27
  • a Proteins related to aromatic compound degradation were induced in cells grown with benzoate as the sole carbon source. The proteomes of CNB-1 cells grown on succinate were used for reference. The 2-DE gel images of proteomes from CNB-1 cells grown on benzoate and succinate are provided in Fig. S1 in the supplemental material. For all comparisons, P was <0.05; i.e., the spot was found only on gels where CNB-1 was grown on benzoate as opposed to succinate. Induction was observed for each protein.

  • b Mascot protein score among the fractions where the protein was identified.

  • c Number of spectra matched to the protein.

  • d Protein sequence coverage for the fraction.

  • e From a Mascot search.

  • f MW, molecular weight (in thousands).