TABLE 1

Bacterial strains and vectors

Strain or vectorApplication and/or characteristic(s)Reference and/or source
Strains
    E. coli
        DH5αHost for cloning; fhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR1747
        BW29427Donor for conjugation; thrB1004 pro thi rpsL hsdSlacZ ΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir]traK. A. Datsenko and B. L. Wanner (Purdue University), unpublished
    M. gryphiswaldense
        MSR-1 R/Swt21
        FM019mamC-mCherryThis work
        FM021mamC-egfpThis work
        FM022mCherry-mamKThis work
        FM025ΔphbCABThis work
        FM046ΔphbCAB mamC-egfpThis work
        FM047ΔphbCAB mamC-mCherryThis work
        FM048ΔphbCAB mCherry-mamKThis work
Vectors
    pK19 mob GIIBackbone for pORFM suicide vectors; npt mobRK2 pMB-1 replicon36; GenBank accession no. AF012346
    pJET 1.2/bluntCloning vector; blaThermo Scientific
    pAP160Source of tetRA. Pollithy, unpublished
    pAP173Source of Ptet and terminator sequencesA. Pollithy, unpublished
    pOR014Construction vector for pORFM GalK; npt terminator mobRK2This work
    pOR025aIntermediate for pORFM GalK construction; ter npt mobRK2 tetRThis work
    pORFM GalKGeneral backbone vector for GalK counterselection; npt galK tetR mobRK2This work
    pORFM bluGeneral backbone vector for GalK counterselection, blue-white screening; lacZα npt galK tetR mobRK2This work
    pFM234phbCAB deletion; npt galK tetR mobRK2This work
    pFM236mamC-egfp chromosomal fusion; npt galK tetR mobRK2This work
    pFM237mamC-mCherry chromosomal fusion; npt galK tetR mobRK2This work
    pFM245mCherry-mamK chromosomal fusion; npt gal, tet, mobRK2This work