TABLE 2

Comparison of Aendpoint observed after 24 h of aerobic or anaerobic incubation between H11 cells harvested during stationary phase (SP) and after starvation at 4°C

Chemical classSubstrateAendpointa
AerobicAnaerobic
SPStarvedSPStarved
Ethylene glycolsTween 200.5100.176b
Tween 400.1130.093
Tween 800.3520.130b
Glycosidesα-Methyl-d-glucosidec0.6200.5630.1870.157
β-Methyl-d-glucoside0.141b
Monosaccharidesd-Fructose0.4840.4600.2780.188b
d-Galactose0.7210.554b0.134b
d-Galacturonic acidc0.6740.7160.3120.274
d-Gluconic acid0.7540.6190.4020.212b
d-Mannose0.6730.504b0.2640.122b
d-Ribose0.5550.5970.191b
d-Xylose0.3570.4840.230b
l-Arabinose0.3350.3750.2040.096b
l-Fucosec0.4860.5080.2050.134
N-Acetyl-d-glucosamine0.5980.4520.4110.172b
N-Acetyl-β-d-mannosamine0.0820.091b
α-d-Glucose0.5320.3680.3390.091b
Oligosaccharidesd-Melibiose0.6990.552b0.2600.144b
d-Trehalose0.6450.496b0.2340.215
Lactulose0.3120.142b
Maltose0.7580.5250.3060.142b
Maltotriose0.6510.4220.3180.220b
Sucrose0.6190.5940.2700.109b
α-d-Lactose0.8880.5330.3630.263b
Sugar alcoholsd-Mannitol0.7180.440b0.271b
Dulcitol0.138b
Glycerol0.2330.555d
Sugar phosphatesd,l-α-Glycerol-phosphate0.340d0.112b
Fructose-6-phosphatec0.6650.5200.3350.293
Glucose-1-phosphatec0.7500.6340.3030.233
Glucose-6-phosphate0.6260.6030.4720.256b
Acetic acidsAcetic acid0.3440.188b0.178b
Dicarboxylic acidsBromo succinic acid0.1240.104b
d,l-Malic acid0.2790.374
d-Malic acid0.4510.299b0.201b
Fumaric acid0.2530.2860.186b
l-Malic acid0.3380.3240.184b
Monomethyl succinate0.165b
Succinic acid0.2290.333
Hydroxy acidsl-Lactic acid0.6650.5150.132b
Keto acidsMethyl pyruvate0.7990.4560.2280.093b
Pyruvic acidc0.5330.5060.3130.253
PropionatesPropionic acid0.111
Sugar acidsd-Glucuronic acidc0.7270.6090.2490.175
Glucuronamide0.324d0.1350.083
l-Galactonic acid-γ-lactonec0.7330.6120.2420.185
Mucic acid0.117b
Fatty acidsFormic acid0.195b
Nucleosides2-Deoxyadenosine1.1600.623b0.2560.164b
Adenosine0.8790.561b0.2270.216
Inosine0.9580.629b0.2380.279
Thymidinec0.6690.6590.3400.321
Uridine0.7940.5570.3270.127b
Amino acidsd-Alanine0.2530.2250.168b
Glycine0.107b
Glycyl-l-glutamic acid0.110b
Glycyl-l-proline0.141b
l-Alanine0.1970.2100.126b
l-Alanyl-glycine0.289d
l-Asparagine0.5360.428
l-Aspartic acid0.4070.382
l-Leucine0.203b
l-Phenylalanine0.111b
l-Proline0.107b
l-Pyroglutamic acid0.117b
l-Serine0.3750.610
  • a Results are the averages from duplicates. —, substrate was tested but not utilized.

  • b The value for stationary-phase cells is significantly greater than that for starved cells (P < 0.05).

  • c Utilization did not differ significantly between stationary-phase and starved cells regardless of the atmospheric growth condition.

  • d The value for stationary-phase cells is significantly less than that for starved cells (P < 0.05).