TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant feature(s)Reference or sourcea
E. coli strains
    XL1-bluesupE44 hsdR17 recA1 gyrA96 thi relA1 lac F′ [proAB+ laclq lacZΔM15 Tn10(Tetr)]Stratagene
    XL1-blue/pBC1.2-nanAXL1-blue containing pBC1.2-nanAThis study
    EC101Cloning host; repA+ kmr82
    EC101/pNZ-M.BbrII+M.BbrIIIEC101 harboring pNZ8048 derivative containing bbrIIM and bbrIIIM45
L. lactis strains
    NZ9000MG1363, pepN::nisRK, nisin-inducible overexpression host56
    NZ9000/pNZ-nanANZ9000 containing pNZ-nanAThis study
    NZ9000/pNZ-nanKNZ9000 containing pNZ-nanKThis study
    NZ9000/pNZ-nanENZ9000 containing pNZ-nanEThis study
    NZ9000/pNZ-nagA2NZ9000 containing pNZ-nagA2This study
    NZ9000/pNZ-nagB1NZ9000 containing pNZ-nagB1This study
    NZ9000/pNZ44-nanANZ9000 containing pNZ44-nanAThis study
B. breve strains
    UCC2003Isolate from nursling stool sample47
    UCC2003-nanApORI19-tet-nanA insertion mutant of UCC2003This study
    UCC2003-nanKpORI19-tet-nanK insertion mutant of UCC2003This study
    UCC2003-nanBpORI19-tet-nanB insertion mutant of UCC2003This study
    UCC2003-nanCpORI19-tet-nanC insertion mutant of UCC2003This study
    UCC2003-nagA2pORI19-tet-nagA2 insertion mutant of UCC2003This study
    UCC2003-nanA/pBC1.2-nanApORI19-tet-nanA insertion mutant of UCC2003 harboring complementation construct pBC1.2-nanAThis study
    JCM7017Isolate from human fecesJCM
    JCM7019Isolate from adult fecesJCM
    NCFB2257Isolate from infant intestineNCFB
    NCFB2258Isolate from infant intestineNCFB
    NCIMB8815Isolate from human fecesNCIMB
    NIZO658Isolate from human fecesNIZO
    LMG13208Isolate from infant intestineLMG
    UCC2007Isolate from nursling stool sampleUCC
    UCC2005Isolate from infant intestineUCC
    NCTC11815Isolate from infant intestineNCTC
    461BIsolate from infant and adult fecesPRL
    689BIsolate from infant feces62
    12LMother's milk62
B. bifidum PRL2010Isolate from infant feces64
Plasmids
    pAM5pBC1-puC19-Tcr54
    pNZ44pNZ8048 containing constitutive p44 promoter from lactococcal chromosome53
    pNZ44-nanApNZ44 harboring nanA downstream of p44 promoterThis study
    pBC1.2pBC1-pSC101-Cmr54
    pBC1.2-nanApBC1-pSC101-Cmr harboring nanA downstream of p44 promoterThis study
    pORI19Emr, RepA, ori+, cloning vector82
    pORI19-tet-nanAInternal 301-bp fragment of nanA and tetW cloned in pORI19This study
    pORI19-tet-nankInternal 370-bp fragment of nanK and tetW cloned in pORI19This study
    pORI19-tet-nanBInternal 504-bp fragment of nanB and tetW cloned in pORI19This study
    pORI19-tet-nanCInternal 355-bp fragment of nanC and tetW cloned in pORI19This study
    pORI19-tet-nagA2Internal 402-bp fragment of nagA2 and tetW cloned in pORI19This study
    pNZ8150Cmr, nisin-inducible translational fusion vector55
    pNZ-nanACmr, pNZ8150 derivative containing translational fusion of nanA-containing DNA fragment to nisin-inducible promoterThis study
    pNZ-nanKCmr, pNZ8150 derivative containing translational fusion of nanK-containing DNA fragment to nisin-inducible promoterThis study
    pNZ-nanECmr, pNZ8150 derivative containing translational fusion of nanE-containing DNA fragment to nisin-inducible promoterThis study
    pNZ-nagA2Cmr, pNZ8150 derivative containing translational fusion of nagA2-containing DNA fragment to nisin-inducible promoterThis study
    pNZ-nagB1Cmr, pNZ8150 derivative containing translational fusion of nagB1-containing DNA fragment to nisin-inducible promoterThis study
  • a JCM, Japanese Collection of Microorganisms; NCFB, National Collection of Food Bacteria; NCIMB, National Collection of Industrial and Marine Bacteria; NIZO, Netherlands Institute for Dairy Research; LMG, Belgian Coordinated Collection of Microorganisms; UCC, University College Cork; NCTC, National Collection of Type Cultures; PRL, Culture Collection of Probiogenomics, University of Parma.