TABLE 1

Genetic diversity and frequency distribution of 130 C. jejuni isolates from humans, peas, and wild birds from the outbreake,f

Sequence typeClonal complexaMLST allelic profilebPFGE profilecPenner serotypedNo. of isolates
HumanWild birdPeaTotal
aspAglnAgltAglyApgmtktuncASmaIKpnIOutbreakSporadic
Human, pea, and wild bird clusters
    ST-5061* (cluster A)UA1038761423394491DBRS 16.1283DBRK02.0549HS19112
    ST-5059* (cluster B)UA10312549577313DBRS 16.0127DBRK02.0546HS85117
    ST-5048* (cluster C)UA10383106419574431DBRS 16.1256DBRK02.0522HS18347
    ST-137 (cluster D)ST-45471044271DBRS 16.0749DBRK02.0524HS15549
    ST-5049* (cluster E)UA18017232247391803UnrestrictedDBRK02.0548HS29, HS212237
Pea and wild bird isolates
    ST-5047*UA18038995475414423549
    ST-5057*UA13893254225411806459
    ST-5050*UA178795635488166
    ST-5056*UA178385951295451804866
    ST-5052*UA131323420394896415
    ST-5055*UA1028027749394443655
    ST-5053*UA129562472400147134
    ST-5054*UA28538432442154244531233
    ST-5073*UA17883274954428633
    ST-5058*UA1234905434436112
    ST-5051*UA1038510612939444311
    ST-5060*UA1038795423397111
    ST-5072*UA290389541839441611
    ST-5074*UA103866141939451111
Human outbreak-associated isolates
    ST-5062*†UA287634424394446172HS4189
HS81
    ST-468UA102506291734533
    ST-5069*UA10661427548447633
    ST-5063*UA103081064757447122
    ST-5065*UA1172542639231622
    ST-5064*UA1829542554644831411
    ST-5067*UA286172542639443611
    ST-5066*UA107427647394495122
    ST-45ST-454710417122
    ST-42ST-42123459311
    ST-48ST-48241271511
Human sporadic isolates
    ST-5066*UA107427647394495111
    ST-45ST-454710417133
    ST-42ST-42123459311
    ST-48ST-48241271511
    ST-19ST-21215321511
    ST-52ST-52925210223611
    ST-353ST-35371752103611
    ST-403ST-40310271619105711
    ST-508ST-5081660241228111
    ST-572ST-2066245221511
    ST-991ST-69237525726107292311
    ST-2875UA22944810255711
    ST-5068*ST-4212345931511
    Total43163140130
  • a UA, unassigned status for a given sequence type.

  • b Newly identified alleles are shown in bold.

  • c PFGE profiles for the restriction enzymes SmaI and KpnI for the five identified clusters (A to E).

  • d Penner serotyping was performed on representatives of human and environmental isolates from clusters A to E and on all ST-5062 isolates, including one from a GBS case.

  • e *, novel STs; †, ST associated with one case of Guillain-Barré syndrome; ‡, overlapping novel alleles found in human and environmental isolates.

  • f The numbers of alleles per locus (% novel) were 14 (28.5), 23 (26), 22 (22.7), 24 (41.6), 22 (31.8), 24 (41.6), and 16 (25) for the aspA, glnA, gltA, glyA, pgm, tkt, and uncA genes, respectively.