TABLE 2

Numbers of OTUs for each sample, according to primer set and DNA extraction method, determined at two levels of dissimilarity (3% and 10%)

SampleExtraction methodaNo. of OTUs with primer set (3%)No. of OTUs with primer set (10%)
BacteriaArchaeaBacteriaArchaea
Bact1Bact2Arch1Arch2Bact1Bact2Arch1Arch2
Dive 1758 CT8 SC1 (cold seep surface layer; gray mat)Zhou171182202165856711332
Kit18517217511387627626
Dive 1758 CT8 SC6 (cold seep bottom layer; gray mat)Zhou2682782961361349613221
Kit2432863021451288811831
Dive 1766 CT3 SH1 (hydrothermal vent surface layer; white mat)Zhou (replicate 1)2432691992571371049491
Kit (replicate 1)208240181222110988978
Zhou (replicate 2)2022401791991199410669
Kit (replicate 2)2312461732191301029173
  • a Zhou, method of Zhou et al. (12).