TABLE 1

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    S. pneumoniae
        D39Serotype 2 strain, cps2Laboratory of P. Hermans
        ΔccpA mutantD39 ΔccpA; Specr3
        MA100D39 ΔlacR; SpecrThis study
        MA101D39 lacT null mutantThis study
        MA102D39 ΔbgaA::PlacA-lacZ; TetrThis study
        MA103MA100 ΔbgaA::PlacA-lacZ; TetrThis study
        MA104MA101 ΔbgaA::PlacA-lacZ; TetrThis study
        MA105D39 ΔbgaA::PlacT-lacZ; TetrThis study
        MA106MA100 ΔbgaA::PlacT-lacZ; TetrThis study
        MA107MA101 ΔbgaA::PlacT-lacZ; TetrThis study
        MA108D39 ΔbgaA::PgalK-lacZ; TetrThis study
        MA109D39 ΔccpA::PlacT-lacZ; TetrThis study
    E. coli EC1000Kmr; MC1000 derivative carrying a single copy of the pWV1 repA gene in glgBLaboratory collection
Plasmids
    pPP2Ampr Tetr; promoterless lacZ; used for replacement of bgaA with the promoter-lacZ fusion; derivative of pPP147
    pORI280Ermr; ori+ ΔrepA; deletion derivative of pWV01; constitutive lacZ expression from the P32 promoter71
    pORI38Specr; ori+ ΔrepA; deletion derivative of pWV0171
    pMA101pPP2 PlacA-lacZThis study
    pMA102pPP2 PlacT-lacZThis study
    pMA103pPP2 PgalK-lacZThis study