TABLE 2

Binding and expression of BDNF mutants

CharacteristicValuea for:
Wild typeDE Rd1DE Rd2
T4T5B2B5P1P3P5P10K3K6K8
Expressionb1.0 ± 0.11.4 ± 0.11.4 ± 0.11.4 ± 0.12.4 ± 0.15.0 ± 0.74.2 ± 0.44.1 ± 0.25.2 ± 0.45.1 ± 0.13.1 ± 0.33.4 ± 0.1
Specific binding activity for:
    TrkBc1.0 ± 0.13.1 ± 0.13.9 ± 0.32.0 ± 0.31.5 ± 0.12.7 ± 0.32.6 ± 0.32.7 ± 0.12.4 ± 0.13.0 ± 0.12.9 ± 0.24.0 ± 0.3
    p75dNone1.6 ± 0.11.0 ± 0.1None0.6 ± 0.15.0 ± 0.93.4 ± 0.35.9 ± 0.24.7 ± 0.52.9 ± 0.13.4 ± 0.33.4 ± 0.3
  • a Fold change, expressed as means ± standard deviations for triplicate independent transformants. Statistically significant differences were found (P, <0.05) for full-length expression and TrkB binding activity between all mutants and wild-type BDNF. Statistically significant differences were also found (P, <0.05) for p75 binding activity between T5 and all other mutants. Statistical evaluation was performed using an unpaired, two-tailed Student t test.

  • b Full-length expression on the yeast surface as determined by c-myc epitope labeling and flow cytometry and normalized to that of wild-type BDNF.

  • c As determined by flow cytometry and normalized to the TrkB binding activity of wild-type BDNF.

  • d As determined by flow cytometry and normalized to the p75 binding activity of mutant T5.