TABLE 2

Purification of HADs from Sphingomonas sp. strain TDK1 and Sphingobium sp. strain TCM1

StepTotal activityc (μmol · min−1)Total protein (mg)Sp act (μmol · min−1 · mg−1)Yield (%)Purification (fold)
TDK-HADa
    Crude extract32.63430.09501001.00
    Ammonium sulfate fractionation26.73040.087781.80.924
    Butyl Sepharose4.6721.10.22214.32.33
    SP Sepharose1.360.6861.984.1620.8
    Sephacryl S-2000.4610.1892.441.4125.6
TCM-HADb
    Crude extract2194690.4701001.00
    Ammonium sulfate fractionation2673890.6901221.50
    SP Sepharose66.09.956.6030.014.0
    Phenyl Sepharose27.04.286.4012.513.6
  • a Starting material was 40.0 g of wet cells.

  • b Starting material was 36.7 g of wet cells.

  • c The activity of TDK-HAD and TCM-HAD was assayed using TDCPP and TCEP as a substrate, respectively.