TABLE 1

Bacterial strains, plasmids, and primers used in this study

Strain, plasmid, or primerDescription or sequenceSource
Strains
    P. nitroreducens BStrain that cleaves BAC to BDMAThis study
    E. coli NEB 5αHost strain for plasmid constructionNEB
    E. coli Rosetta 2(DE3)Host strain for enzyme overexpressionNovagen
Plasmids
    pET21aAmpr; overexpression vectorNovagen
    pET-KJ911918Ampr; pET-21a containing the amine oxidase gene from P. nitroreducens strain BThis study
Primers
    AMO-FPCR amplification of 1,458-bp open reading frame of the amine oxidase gene from P. nitroreducens strain B; TTTGAATTCATGGCCGATGAAAAAGACGACThis study
    AMO-RPCR amplification of 1,458-bp open reading frame of the amine oxidase gene from P. nitroreducens strain B; TTTAAGCTTTCATCCCAGCAGCThis study