TABLE 2

Microarray expression ratios comparing the relative expressions of polyamine transporter genes in furfural-resistant mutants (EMFR9 and EMFR35) and the parent (LY180)a

TransporterGeneLocus tagbGene expression ratio (mutant/LY180)
EMFR9 (0 mM furfural)EMFR9 (5 mM furfural)EMFR35 (0 mM furfural)EMFR35 (15 mM furfural)
ABC transporter, spermidine/putrescinepotAb11260.81.00.82.2
potBb11251.11.30.73.0
potCb11240.91.10.53.1
potDb11230.81.31.21.0
ABC transporter, putrescinepotFb08541.00.70.91.4
potGb08551.00.71.01.2
potHb08560.80.81.21.1
potIb08570.80.91.31.2
ABC transporter, spermidine/putrescineydcSb14401.01.01.31.1
ydcTb14411.01.31.51.6
ydcUb14420.91.20.91.4
ydcVb14430.91.11.01.2
Putrescine symporterplaPb20142.41.91.710
Putrescine symporterpotEb06921201001.00.8
Putrescine symporterpuuPb12960.91.1750590
Spermidine antiportermdtJb41321.31.11.00.3
mdtIb15991.71.51.01.9
Cadaverine symportercadBb41320.61.40.80.7
  • a Comparisons were made using cells grown in the presence and absence of furfural. Two genes that are highly upregulated are shown in bold.

  • b Locus tags are based on E. coli K-12 strain MG1655 classification (GenBank accession number NC_000913.3).