TABLE 1

Fermentor nutrient medium

IngredientaConcn
Peptone water2.0 g/liter
Yeast extract2.0 g/liter
NaCl0.1 g/liter
K2HPO40.04 g/liter
KH2PO40.04 g/liter
MgSO4 · 7H2O0.01 g/liter
CaCl2 · 6H2O0.01 g/liter
NaHCO32.0 g/liter
Cysteine-HCl0.5 g/liter
Bile salts0.5 g/liter
Heminb0.05 g/liter
Tween 802.0 ml/liter
Vitamin K10.01 ml/liter
  • a All chemicals were obtained from Sigma.

  • b Hemin solution consisted of 0.5 g hemin in 25 ml 1 M NaOH.